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Image Search Results
Journal: Journal of Inflammation (London, England)
Article Title: Comparative effects of the herbal constituent parthenolide (Feverfew) on lipopolysaccharide-induced inflammatory gene expression in murine spleen and liver
doi: 10.1186/1476-9255-2-6
Figure Lengend Snippet: IL-6 protein production in sera following parthenolide and LPS co-treatment. Female B6C3F1 mice were co-treated with parthenolide (5 mg/kg, i.p.) or 50 μl DMSO and LPS (1 mg/kg, i.p.) or 100 μl water. After 90 minutes, blood was collected and serum analyzed for IL-6 by ELISA. The letter (a) indicates a significant difference compared to vehicle and parthenolide controls; (b) indicates a significant difference compared to LPS. Data are mean ± SEM (n = 16, controls n = 4), and is a combination of 4 separate experiments.
Article Snippet: IL-6 analysis was performed using purified and
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Journal of Inflammation (London, England)
Article Title: Comparative effects of the herbal constituent parthenolide (Feverfew) on lipopolysaccharide-induced inflammatory gene expression in murine spleen and liver
doi: 10.1186/1476-9255-2-6
Figure Lengend Snippet: IL-6 mRNA expression levels in spleen and liver following parthenolide and LPS co-treatment. Female B6C3F1 mice were co-treated with parthenolide (5 mg/kg, i.p.) or 50 μl DMSO and LPS (1 mg/kg, i.p.) or 100 μl water. Spleen and liver were collected after 90 minutes and total RNA was extracted and subjected to real-time, one-step RT-PCR using TaqMan primers and probes. IL-6 mRNA levels were normalized using 18S rRNA and related to spleen control values. (a) indicates a significant difference compared to vehicle and parthenolide controls; (b) indicates a significant difference compared to LPS. Data are mean ± SEM (n = 16, controls n = 4), and is a combination of 4 separate experiments.
Article Snippet: IL-6 analysis was performed using purified and
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction